Ion channels are large transmembrane proteins which, besides transporters, controlling the movement of ions between the two sides of the cell membrane. Their opening and closing could be determined by the membrane potential, neurotransmitters, external stimuli (receptor potential) or the internal state of the cell through second messengers or signal transduction mechanisms. We are using whole cell patch clamp method to study the effect of drugs on ion channels. We are using primary cultures of chicken neurons and cardiac cells and C2C12 mouse skeletal muscle line for this purpose.

5 days old chicken neuron culture and recorded whole-cell currents

4 days old differentiated C2C12 cells. Contractions (every 5s, difficult to see) and action potentials